Posted by: Indonesian Children | August 15, 2009

Expression Levels of TLRs Involved in Viral Recognition in PBMCs from HIV-1-Infected Patients Failing Antiretroviral Therapy

Free Abstract     Article (References)     Article (PDF 193 KB)     


Original Paper

Expression Levels of TLRs Involved in Viral Recognition in PBMCs from HIV-1-Infected Patients Failing Antiretroviral Therapy
Carolina Scagnolaria, Carla Selvaggia, Luisa Chiavuzzoa, Teresa Carbonea, Lorenzo Zaffirib, Gabriella d’Ettorreb, Enrico Girardic, Ombretta Turriziania, Vincenzo Vullob, Guido Antonellia

aVirology Section, Department of Experimental Medicine, and
bDepartment of Infectious Diseases, Policlinico Umberto I, Sapienza University, and
cNational Institute for Infectious Diseases L. Spallanzani, Rome, Italy

Address of Corresponding Author

Intervirology 2009;52:107-114 (DOI: 10.1159/000218082)



 goto top of page Key Words

  • Human immunodeficiency virus
  • Pathogenesis
  • Pattern recognition receptors
  • Toll-like receptors
  • Viremia



 goto top of page Abstract

Objective: The aim of this study was to evaluate whether gene expression of Toll-like receptor (TLR)-3, TLR4, TLR7 and TLR9 was impaired in patients with chronic HIV-1 infection who were failing to respond to antiretroviral therapy. Methods: Transcripts encoding TLRs were assayed by quantitative real time RT-PCR on peripheral blood mononuclear cells derived from patients with HIV-1 infection who were responding or not responding to antiretroviral therapy and healthy control subjects. Results: Chronic HIV-1-infected patients who failed to respond to therapy showed reduced expression of TLRs 3, 4 and 9, together with increased expression of TLR7, as compared to healthy subjects. Moreover, a trend towards a higher expression of TLR3 and TLR9 was observed in responder patients compared with non-responders. In addition, we found lower levels of TRLs 3, 7 and 9 in patients with high levels of HIV-1 RNA compared to those with lower levels of viremia. Conclusions: These findings demonstrate that in chronic HIV-1-positive patients who were failing to respond to the therapy, there were substantial changes in TLRs expression. This is likely to be an important determinant of the clinical course of HIV-1 infection.

Copyright © 2009 S. Karger AG, Basel

 

Supported by

FIGHT AGAINST  AIDS, SAVE  INDONESIAN CHILDREN

YUDHASMARA FOUNDATION

JL TAMAN BENDUNGAN ASAHAN 5 JAKARTA PUSAT, JAKARTA INDONESIA 10210

PHONE :62 (021) 70081995 – 5703646

Email : judarwanto@gmail.com 

https://childrenhivaids.wordpress.com/

 

Clinical and Editor in Chief :

DR WIDODO JUDARWANTO

email : judarwanto@gmail.com,

 

Copyright © 2009,  FIGHT AGAINST  AIDS, SAVE  INDONESIAN CHILDREN  Information Education Network. All rights reserved.


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Categories

%d bloggers like this: