Posted by: Indonesian Children | August 15, 2009

Effect of intrauterine HIV-1 exposure on the frequency and function of uninfected newborns’ dendritic cells

Paula A. Velillaa, b, c, Carlos J. Montoyaa, Alvaro Hoyosa, Maria E. Morenoa, Claire Chougnetb, c, 1 and Maria T. Rugelesa, Corresponding Author Contact Information, 1, E-mail The Corresponding Author

aGroup of Immunovirology, School of Medicine, University of Antioquia, Medellin, Colombia

bDivision of Molecular Immunology, Cincinnati Children’s Hospital Research Foundation, Cincinnati, OH, USA

cDepartment of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA

Received 30 August 2007; 

accepted 10 November 2007. 

Available online 16 January 2008.

 

Abstract

Immaturity of the neonatal immune system is considered an underlying factor for enhanced severity of infections in newborns. Functional defects of neonatal antigen-presenting cells lead to defective T-cell responses. T cells from uninfected neonates exposed in utero to HIV-1 (EU) exhibit phenotypic and functional alterations; however, the function of their circulating dendritic cells (DCs) has not been characterized. We hypothesized that an HIV-1-infected maternal environment may influence the infants’ DC number, phenotype and function. EU exhibited a higher percentage of myeloid DCs (mDCs) than unexposed neonates, although this frequency remained lower than that observed in adults. Plasmacytoid DC (pDC) frequencies were similar in all groups, although both groups of infants tended to have lower frequencies than adults. After LPS stimulation, mDCs from EU up-regulated CD80, CD86 and B7-H1, whereas mDCs from unexposed infants upregulated B7-H1, but not CD80/CD86, and adult mDCs up-regulated mainly CD80 and CD86. IFN-α production was similar in all groups, indicating a normal pDC function. Therefore, in utero exposure to HIV-1 induces quantitative and qualitative changes in neonatal DCs, particularly in mDCs, which might be associated with alterations observed in T cells from these EU.

Keywords: HIV-1; Neonates; Dendritic cells; CD80; CD86; B7-H1; IFN-α; CpG ODN; LPS

Abbreviations: APCs, Antigen-presenting cells; CB, cord blood; CBMC, cord blood mononuclear cell; CpG ODN, oligodeoxyribonucelotides with CpG motifs; DC, dendritic cells; EU, HIV-1-uninfected neonates born to HIV-1-infected mothers; MC, mononuclear cell; mDC, myeloid dendritic cell; MFI, mean fluorescence intensity; pDC, plasmacytoid dendritic cell; PB, peripheral blood; UN, unexposed neonates (born to HIV-1 negative mothers)

Article Outline

Introduction
Materials and methods
Study population
Reagents
Culture of mononuclear cells
Flow cytometry
Cytokine determination
Statistical analysis
Results
Frequency of dendritic cells
Expression of accessory molecules
Up-regulation of accessory molecule expression induced by TLR agonists
Secretion of IFN-α in response to stimulation with influenza virus or CpG ODN
Discussion
Acknowledgements
References 

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Copyright © 2009,  FIGHT AGAINST  AIDS, SAVE  INDONESIAN CHILDREN  Information Education Network. All rights reserved.

 


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